Gene name |
SPAC513.03 |
Gene ID |
01/A07 |
Gene synonyms/obsolete |
mfm2 |
Gene product |
M-factor precursor 2;
Prenyl group binding site (CAAX box); pheromone; no apparent
orthologs |
Entry clone |
Cloned |
ORF length (unspliced) |
177 |
ORF length (spliced) |
135 |
Entry clone length |
177 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC513.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACTCCATTGCAACTAA |
Rev primer name |
SPAC513.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCAATTACACTATATATT |
Amino acid length |
44 |
Molecular weight |
4.8 |
Isoelectric point (calc.) |
8.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |