Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC11H11.05c
Gene ID 01/A12
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein; correlation score and good intron; has transcript profile on microarray
Entry clone Cloned
ORF length (unspliced) 227
ORF length (spliced) 180
Entry clone length 227
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC11H11.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGGATAAATACATTTT
Rev primer name SPAC11H11.05.Rv
Rev primer SEQ AGAAAGCTGGGTACGAACTTTTTTTTTTATTT
Amino acid length 59
Molecular weight 6.7
Isoelectric point (calc.) 9.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) SPB; nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss, DeltaVision

Image information
YFP 3 images) See all images

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