Gene name |
SPAC1687.14c |
Gene ID |
01/B02 |
Gene synonyms/obsolete |
|
Gene product |
low similarity to
caltractin; centrin; EF-hand motif; no apparent Sc
ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
231 |
ORF length (spliced) |
|
Entry clone length |
231 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1687.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGTCAGTACTAAACG |
Rev primer name |
SPAC1687.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGATATATGAATCCACAAT |
Amino acid length |
76 |
Molecular weight |
8.7 |
Isoelectric point (calc.) |
4.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
SPB;
nucleus>cytosol; nuclear dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss, DeltaVision
|