| Gene name |
SPAC19B12.04 |
| Gene ID |
01/B04 |
| Gene synonyms/obsolete |
rps3001; rps30-1 |
| Gene product |
40S ribosomal protein
S30 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
238 |
| ORF length (spliced) |
186 |
| Entry clone length |
238 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC19B12.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGCAAGGTTCACGGATC |
| Rev primer name |
SPAC19B12.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGATGAAGGATTCATTCTT |
| Amino acid length |
61 |
| Molecular weight |
6.9 |
| Isoelectric point (calc.) |
12.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol>nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleolus>nucleus=cytosol |
| Microscope used for
observation |
Zeiss |