Gene name |
SPBC21B10.11 |
Gene ID |
01/B12 |
Gene synonyms/obsolete |
|
Gene product |
dolichol phosphate
mannose regulatory protein (Dpm2-like); 1TM |
Entry clone |
Cloned |
ORF length (unspliced) |
262 |
ORF length (spliced) |
219 |
Entry clone length |
262 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
22G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC21B10.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTGTTTATATCTCGAC |
Rev primer name |
SPBC21B10.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAAGTCCGACTTCTTTTTA |
Amino acid length |
72 |
Molecular weight |
8.2 |
Isoelectric point (calc.) |
9.1 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIGTFVSLLMI |
Localization (YFP) |
ER; cytoplasmic
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss, DeltaVision
|