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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC417.02
Gene ID 01/C11
Gene synonyms/obsolete hos3
Gene product required for growth under high osmotic stress; no apparent orthologs
Entry clone Cloned
ORF length (unspliced) 285
ORF length (spliced)
Entry clone length 285
No. of intron 0
Sequence status Finished
Sequence results 183T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC417.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGTCGTTCTACCATTGT
Rev primer name SPCC417.02.Rv
Rev primer SEQ AGAAAGCTGGGTACATAGGAACGTCTTCTTCC
Amino acid length 94
Molecular weight 10.3
Isoelectric point (calc.) 10.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 1 images) See all images

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