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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC6G9.13c
Gene ID 01/E06
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan; has transcript profile on microarray
Entry clone Cloned
ORF length (unspliced) 306
ORF length (spliced)
Entry clone length 306
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC6G9.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCATTTATTAACTACGAC
Rev primer name SPAC6G9.13.Rv
Rev primer SEQ AGAAAGCTGGGTATACTGATAGACAGAACTTA
Amino acid length 101
Molecular weight 11.8
Isoelectric point (calc.) 6.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 1 images) See all images

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