Gene name |
SPAC1834.03c |
Gene ID |
01/F02 |
Gene synonyms/obsolete |
h4.1; hhf1 |
Gene product |
histone H4 |
Entry clone |
Cloned |
ORF length (unspliced) |
312 |
ORF length (spliced) |
|
Entry clone length |
312 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
9C:T |
Comments |
Identical to
SPBC8D2.03c and SPBC1105.12. |
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1834.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGGTCGTGGTAAAGG |
Rev primer name |
SPAC1834.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACCACCGAAACCATAAATG |
Amino acid length |
103 |
Molecular weight |
11.4 |
Isoelectric point (calc.) |
11.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |