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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC550.06c
Gene ID 01/F06
Gene synonyms/obsolete hsp10
Gene product heat shock protein (10 kD) mitochondrial groES chaperonin family
Entry clone Cloned
ORF length (unspliced) 315
ORF length (spliced)
Entry clone length 315
No. of intron 0
Sequence status Finished
Sequence results 295T:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC550.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTACCAAACTGAAATC
Rev primer name SPCC550.06.Rv
Rev primer SEQ AGAAAGCTGGGTATTCCTTGATGATAGCCAAC
Amino acid length 104
Molecular weight 11.2
Isoelectric point (calc.) 9.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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