| Gene name |
SPBC21C3.16c |
| Gene ID |
01/F09 |
| Gene synonyms/obsolete |
spt4 |
| Gene product |
zinc finger protein;
transcriptional regulator; involved in transcriptional
regulation; involved in transcription initiation; involved in
DNA repair; involved in DNA damage response; interacts
physically with Spt5p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
318 |
| ORF length (spliced) |
|
| Entry clone length |
318 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
64A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC21C3.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATAAGCTAAATAGGAC |
| Rev primer name |
SPBC21C3.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCCCATGATGTACCATTC |
| Amino acid length |
105 |
| Molecular weight |
11.8 |
| Isoelectric point (calc.) |
8 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |