Gene name |
SPBC27.05 |
Gene ID |
01/G08 |
Gene synonyms/obsolete |
|
Gene product |
very hypothetical
protein; sequence orphan; has transcript profile on microarray
but this could be generated by SPBC27.04 proximal and has the
same expression pattern |
Entry clone |
Cloned |
ORF length (unspliced) |
327 |
ORF length (spliced) |
|
Entry clone length |
327 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC27.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTGCATACAACGGTTTA |
Rev primer name |
SPBC27.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTGATCATAAAGATATTT |
Amino acid length |
108 |
Molecular weight |
12.7 |
Isoelectric point (calc.) |
8.1 |
Signal SEQ |
|
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Zeiss |