Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC18G6.13
Gene ID 02/B03
Gene synonyms/obsolete
Gene product hypothetical protein; similar at the N-terminal to Sp SPCC1682.03C
Entry clone Cloned
ORF length (unspliced) 345
ORF length (spliced)
Entry clone length 345
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC18G6.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGGCTCAAAGTCTCCTG
Rev primer name SPAC18G6.13.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCCTCCATTTCTACATCT
Amino acid length 114
Molecular weight 13.1
Isoelectric point (calc.) 4.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nuclear envelope
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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