Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC869.09
Gene ID 02/C03
Gene synonyms/obsolete
Gene product hypothetical protein; similar to N. crassa conidiation-specific protein 6; no apparent Sc ortholog; similar to Sp SPAC22G7.11C and SPAC11D3.01C; involved in sporulation
Entry clone Cloned
ORF length (unspliced) 351
ORF length (spliced)
Entry clone length 351
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC869.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTGATCCTGCACATAT
Rev primer name SPAC869.09.Rv
Rev primer SEQ AGAAAGCTGGGTACTTAGACTCATCGTCAACT
Amino acid length 116
Molecular weight 12.8
Isoelectric point (calc.) 6.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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