Gene name |
SPAPJ695.01c |
Gene ID |
02/C06 |
Gene synonyms/obsolete |
SPAP695.01c |
Gene product |
33896 domain; possibly
Sp specific; telomeric duplication |
Entry clone |
Cloned |
ORF length (unspliced) |
354 |
ORF length (spliced) |
|
Entry clone length |
354 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
(347~352)CCAAAT:deletion |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAP695.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTATTGCTTTTATACAT |
Rev primer name |
SPAP695.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTGGGATCCAAGTCGCTG |
Amino acid length |
117 |
Molecular weight |
13.2 |
Isoelectric point (calc.) |
3.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLLYICCLFL |
Localization (YFP) |
mitochondrion |
Comments for localization |
aggregates by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |