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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPJ695.01c
Gene ID 02/C06
Gene synonyms/obsolete SPAP695.01c
Gene product 33896 domain; possibly Sp specific; telomeric duplication
Entry clone Cloned
ORF length (unspliced) 354
ORF length (spliced)
Entry clone length 354
No. of intron 0
Sequence status Finished
Sequence results (347~352)CCAAAT:deletion
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAP695.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTATTGCTTTTATACAT
Rev primer name SPAP695.01.Rv
Rev primer SEQ AGAAAGCTGGGTATTTGGGATCCAAGTCGCTG
Amino acid length 117
Molecular weight 13.2
Isoelectric point (calc.) 3.9
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLLYICCLFL
Localization (YFP) mitochondrion
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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