Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC15A10.12c
Gene ID 02/C09
Gene synonyms/obsolete
Gene product hypothetical protein; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 354
ORF length (spliced)
Entry clone length 354
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC15A10.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTAAAACCAAGGTTGGT
Rev primer name SPAC15A10.12.Rv
Rev primer SEQ AGAAAGCTGGGTAAAATATTGACAGTTTTATC
Amino acid length 117
Molecular weight 13.3
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.