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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC31G5.21
Gene ID 02/D08
Gene synonyms/obsolete
Gene product hypothetical protein; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 357
ORF length (spliced)
Entry clone length 357
No. of intron 0
Sequence status Finished
Sequence results 84T:A / 126T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC31G5.21.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGACTACGTTGGTGGTTC
Rev primer name SPAC31G5.21.Rv
Rev primer SEQ AGAAAGCTGGGTAGCCGGGTCCAATTTTAGGC
Amino acid length 118
Molecular weight 13.7
Isoelectric point (calc.) 10.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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