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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3G6.02
Gene ID 02/D11
Gene synonyms/obsolete dss1
Gene product exonuclease complex component; involved in mitochondrial biogenesis; human disease dss1 homolog
Entry clone Cloned
ORF length (unspliced) 362
ORF length (spliced) 216
Entry clone length 362
No. of intron 2
Sequence status Finished
Sequence results 305T:A / 342A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3G6.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTCGTGCGGCCTTACC
Rev primer name SPAC3G6.02.Rv
Rev primer SEQ AGAAAGCTGGGTAATTGGCGGCAACACCCTTC
Amino acid length 71
Molecular weight 8.1
Isoelectric point (calc.) 3.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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