Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC227.17c
Gene ID 02/E06
Gene synonyms/obsolete
Gene product hypothetical protein; YDR512C (Sc homolog) is induced early in meiosis
Entry clone Cloned
ORF length (unspliced) 366
ORF length (spliced)
Entry clone length 366
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC227.17.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATAATTTTAAAGAAAA
Rev primer name SPAC227.17.Rv
Rev primer SEQ AGAAAGCTGGGTATTCACTACTTTTTGGAGAA
Amino acid length 121
Molecular weight 14.5
Isoelectric point (calc.) 8.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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