| Gene name |
SPAC29B12.03 |
| Gene ID |
02/F08 |
| Gene synonyms/obsolete |
spd1 |
| Gene product |
ribonucleotide
reductase (RNR) anchor; degradation is promoted by two
separate CSN-dependent mechanisms; no apparent orthologs;
S-phase inhibitor |
| Entry clone |
Cloned |
| ORF length (unspliced) |
375 |
| ORF length (spliced) |
|
| Entry clone length |
375 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC29B12.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCACAGCAGCAAGCGAGT |
| Rev primer name |
SPAC29B12.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAATCTCTCATTGGTACCC |
| Amino acid length |
124 |
| Molecular weight |
14.2 |
| Isoelectric point (calc.) |
7.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol; a
few nuclear dots; filamentous structures, occasionally |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |