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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1A4.09c
Gene ID 02/F10
Gene synonyms/obsolete SPBC1A4.11c; SPBC1A4.09a
Gene product very hypothetical ORF
Entry clone Cloned
ORF length (unspliced) 375
ORF length (spliced)
Entry clone length 375
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1A4.09a.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCCGAGAAGGATTGGAT
Rev primer name SPBC1A4.09a.Rv
Rev primer SEQ AGAAAGCTGGGTAAGGCGTAAGATTGTCAATT
Amino acid length 124
Molecular weight 14.2
Isoelectric point (calc.) 4.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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