Gene name |
SPAC5D6.08c |
Gene ID |
02/G08 |
Gene synonyms/obsolete |
mes1 |
Gene product |
meiosis II protein;
displays meiosis specific splicing; no apparent orthologs;
undergoes meiosis-dependent mRNA splicing; 5' terminal region
mRNA is crucial for its meiosis-specific splicing |
Entry clone |
Cloned |
ORF length (unspliced) |
381 |
ORF length (spliced) |
306 |
Entry clone length |
381 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC5D6.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAAACACCGACAACAA |
Rev primer name |
SPAC5D6.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGCGCATTGAAAGCAATGGA |
Amino acid length |
101 |
Molecular weight |
11.3 |
Isoelectric point (calc.) |
10.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |