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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2F3.17c
Gene ID 02/H02
Gene synonyms/obsolete lsm6
Gene product small nuclear ribonucleoprotein, F-like; involved in mRNA splicing
Entry clone Cloned
ORF length (unspliced) 387
ORF length (spliced) 228
Entry clone length 387
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2F3.17.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTCTTCTCCTAATGA
Rev primer name SPAC2F3.17.Rv
Rev primer SEQ AGAAAGCTGGGTAATCATCTAAAGCAGAAACA
Amino acid length 75
Molecular weight 8.3
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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