Gene name |
SPAC9G1.03c |
Gene ID |
02/H06 |
Gene synonyms/obsolete |
rpl3001; rpl30;
rpl30-1 |
Gene product |
60S ribosomal protein
L30/L30A |
Entry clone |
Cloned |
ORF length (unspliced) |
389 |
ORF length (spliced) |
330 |
Entry clone length |
389 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
(-7)C:deletion |
Comments |
5' terminus is
frameshifted. |
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC9G1.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCATCTGTGGTTACCAA |
Rev primer name |
SPAC9G1.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCGTCCAAGATGTCAGAG |
Amino acid length |
109 |
Molecular weight |
11.5 |
Isoelectric point (calc.) |
10.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFRVGVLAI |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |