Gene name |
SPAC6F12.04 |
Gene ID |
03/A06 |
Gene synonyms/obsolete |
|
Gene product |
COPI-coated vesicle
associated protein; conserved fungal protein; similar to S.
cerevisiae YDR100W; 4 predicted transmembrane helices;
involved in vesicular transport and membrane fusion (Sc
2-hybrid data) |
Entry clone |
Cloned |
ORF length (unspliced) |
399 |
ORF length (spliced) |
|
Entry clone length |
399 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC6F12.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATAAAACAAAAATGTT |
Rev primer name |
SPAC6F12.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACACTTCATCAACGTTATCA |
Amino acid length |
132 |
Molecular weight |
14.5 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
4 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
Golgi |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal,
DeltaVision |