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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC29A4.03c
Gene ID 03/A08
Gene synonyms/obsolete
Gene product mitochondrial ribosomal protein S9
Entry clone Cloned
ORF length (unspliced) 399
ORF length (spliced)
Entry clone length 399
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC29A4.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTTGATGAAAATGGAAT
Rev primer name SPAC29A4.03.Rv
Rev primer SEQ AGAAAGCTGGGTAACGCTTGACCCATGTGTAT
Amino acid length 132
Molecular weight 14.7
Isoelectric point (calc.) 11
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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