Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1840.10
Gene ID 03/B02
Gene synonyms/obsolete lsm8
Gene product small nuclear ribonucleoprotein complex; involved in mRNA splicing; involved in rRNA processing
Entry clone Cloned
ORF length (unspliced) 402
ORF length (spliced) 285
Entry clone length 402
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1840.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTCTTGCTGATTTTAT
Rev primer name SPCC1840.10.Rv
Rev primer SEQ AGAAAGCTGGGTAATGCACAACGTCAGGTATT
Amino acid length 94
Molecular weight 10.5
Isoelectric point (calc.) 4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.