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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1442.08c
Gene ID 03/B10
Gene synonyms/obsolete cox12
Gene product cytochrome c oxidase polypeptide Vib
Entry clone Cloned
ORF length (unspliced) 410
ORF length (spliced) 252
Entry clone length 410
No. of intron 2
Sequence status Finished
Sequence results (-4)T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1442.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACATTGGTGATATTATC
Rev primer name SPCC1442.08.Rv
Rev primer SEQ AGAAAGCTGGGTAGATAGGGGCCGGGAAGGTT
Amino acid length 83
Molecular weight 10
Isoelectric point (calc.) 7.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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