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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC18.11c
Gene ID 03/C06
Gene synonyms/obsolete
Gene product SET1 complex; non-essential; Dpy-30 domain protein; involved in transcriptional silencing; involved in regulation of chromatin remodelling
Entry clone Cloned
ORF length (unspliced) 414
ORF length (spliced) 330
Entry clone length 414
No. of intron 1
Sequence status Finished
Sequence results 219A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC18.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAAACAGCGCACCTGC
Rev primer name SPCC18.11.Rv
Rev primer SEQ AGAAAGCTGGGTATGAAGCAGAATTTTTACCA
Amino acid length 109
Molecular weight 11.8
Isoelectric point (calc.) 4.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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