Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBC31F10.09c
Gene ID 03/C11
Gene synonyms/obsolete nut2
Gene product RNA polymerase II holoenzyme component; mediator complex subunit; involved in regulating RNA polymerase II activity
Entry clone Cloned
ORF length (unspliced) 417
ORF length (spliced)
Entry clone length 417
No. of intron 0
Sequence status Finished
Sequence results 258A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC31F10.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGATGAAATGAAATC
Rev primer name SPBC31F10.09.Rv
Rev primer SEQ AGAAAGCTGGGTATGAAACTGTAGATTCGACT
Amino acid length 138
Molecular weight 15.6
Isoelectric point (calc.) 4.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol; nuclear envelope
Comments for localization
Effect of LMB on protein localization changed to: nucleus
Microscope used for observation Confocal

Image information
YFP 1 images) See all images
LMB 4 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.