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Schizosaccharomyces pombe Postgenome Database

Gene name SPCP31B10.03c
Gene ID 03/D03
Gene synonyms/obsolete sep10
Gene product general transcriptional regulator; RNA polymerase II accessory protein; mutant defective in cell separation; mutant defective in sexual differentiation; synthetic lethal with sep11
Entry clone Cloned
ORF length (unspliced) 420
ORF length (spliced)
Entry clone length 420
No. of intron 0
Sequence status Finished
Sequence results 277T:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCP31B10.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAACAAAATGGTTACT
Rev primer name SPCP31B10.03.Rv
Rev primer SEQ AGAAAGCTGGGTATTCATTTTCCTTTTTCACG
Amino acid length 139
Molecular weight 16.8
Isoelectric point (calc.) 4.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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