Gene name |
SPAC6F6.05 |
Gene ID |
03/D08 |
Gene synonyms/obsolete |
|
Gene product |
oligosaccharyltransferase (epsilon subunit);
defender against cell death homolog |
Entry clone |
Cloned |
ORF length (unspliced) |
422 |
ORF length (spliced) |
369 |
Entry clone length |
422 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC6F6.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTCCAAAAGTGGATT |
Rev primer name |
SPAC6F6.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCCTAGGAAATTGACAGCA |
Amino acid length |
122 |
Molecular weight |
13.3 |
Isoelectric point (calc.) |
9.5 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
3 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEFCFSSLVL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |