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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC644.05c
Gene ID 03/D10
Gene synonyms/obsolete
Gene product deoxyuridine 5'-triphosphate nucleotidohydrolase; dUTP pyrophosphatase
Entry clone Cloned#
ORF length (unspliced) 423
ORF length (spliced)
Entry clone length 423
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC644.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTTTTTTCGTTCAAAA
Rev primer name SPAC644.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAACACCGGTACTTCCAAAG
Amino acid length 140
Molecular weight 14.8
Isoelectric point (calc.) 7.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>=cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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