Gene name |
SPCC24B10.13 |
Gene ID |
03/D12 |
Gene synonyms/obsolete |
skb5 |
Gene product |
interacts physically
with Shk1p; activator of PAK Shk1; src (SH3) homology
domain |
Entry clone |
Cloned |
ORF length (unspliced) |
423 |
ORF length (spliced) |
|
Entry clone length |
423 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
403T:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC24B10.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGGAAGAGACTGAAGA |
Rev primer name |
SPCC24B10.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATACCTCTAATTTAACAAAC |
Amino acid length |
140 |
Molecular weight |
15.8 |
Isoelectric point (calc.) |
3.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |