Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC16E8.03
Gene ID 03/G01
Gene synonyms/obsolete gna1; spgna1
Gene product glucosamine-phosphate N-acetyltransferase involved in UDP-N-acetylglucosamine synthesis
Entry clone Cloned
ORF length (unspliced) 437
ORF length (spliced) 336
Entry clone length 437
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC16E8.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGAAAGACTTTCATTC
Rev primer name SPAC16E8.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAATAATTGAGTGGCTAGCG
Amino acid length 111
Molecular weight 12.5
Isoelectric point (calc.) 8.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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