Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC31G5.06
Gene ID 03/G03
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 438
ORF length (spliced)
Entry clone length 438
No. of intron 0
Sequence status Finished
Sequence results 291G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC31G5.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAATTAAATAATTTGAA
Rev primer name SPAC31G5.06.Rv
Rev primer SEQ AGAAAGCTGGGTATGAGGAATACGCAGCGGGC
Amino acid length 145
Molecular weight 17
Isoelectric point (calc.) 9.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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