Gene name |
SPBC20F10.09 |
Gene ID |
03/G06 |
Gene synonyms/obsolete |
lsm5 |
Gene product |
U6 snRNA-associated
protein; Sm domain; involved in mRNA splicing |
Entry clone |
Cloned |
ORF length (unspliced) |
438 |
ORF length (spliced) |
243 |
Entry clone length |
438 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC20F10.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGATGACAATTTTACC |
Rev primer name |
SPBC20F10.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCGGGTTTTCCACCAGGA |
Amino acid length |
80 |
Molecular weight |
8.8 |
Isoelectric point (calc.) |
4.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |