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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1682.01
Gene ID 03/H07
Gene synonyms/obsolete qcr9
Gene product ubiquinol cytochrome c reductase complex subunit (cytochrome bc1 complex); respiratory complex III
Entry clone Cloned
ORF length (unspliced) 448
ORF length (spliced) 258
Entry clone length 448
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1682.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTATTCGCTCAGTTTTC
Rev primer name SPCC1682.01.Rv
Rev primer SEQ AGAAAGCTGGGTATTCATCATCCTCAGCATCT
Amino acid length 85
Molecular weight 9.7
Isoelectric point (calc.) 4.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization aggregates
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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