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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC191.10
Gene ID 03/H10
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned#
ORF length (unspliced) 450
ORF length (spliced)
Entry clone length 450
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC191.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCATTCTAGCAGACGGAA
Rev primer name SPCC191.10.Rv
Rev primer SEQ AGAAAGCTGGGTATCTACCTAGAGGCAATTGA
Amino acid length 149
Molecular weight 17.3
Isoelectric point (calc.) 10.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion; cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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