| Gene name |
SPBC2D10.19c |
| Gene ID |
03/H12 |
| Gene synonyms/obsolete |
|
| Gene product |
small hypothetical
protein; confirmed by EST |
| Entry clone |
Cloned |
| ORF length (unspliced) |
452 |
| ORF length (spliced) |
318 |
| Entry clone length |
452 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
376C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC2D10.19.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCCCATAGAAACGATAG |
| Rev primer name |
SPBC2D10.19.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAATGCTGGAGGAGCACCC |
| Amino acid length |
105 |
| Molecular weight |
11.6 |
| Isoelectric point (calc.) |
10.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus>nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|