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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22F3.07c
Gene ID 04/A07
Gene synonyms/obsolete
Gene product ATP synthase G chain
Entry clone Cloned
ORF length (unspliced) 455
ORF length (spliced) 357
Entry clone length 455
No. of intron 2
Sequence status Finished
Sequence results 60C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC22F3.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTTCCACAAGTCGAAT
Rev primer name SPAC22F3.07.Rv
Rev primer SEQ AGAAAGCTGGGTAATGATATGGAGTAATTTTT
Amino acid length 118
Molecular weight 13.6
Isoelectric point (calc.) 10.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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