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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC890.08
Gene ID 04/B08
Gene synonyms/obsolete rpl31
Gene product 60S ribosomal protein L31
Entry clone Cloned
ORF length (unspliced) 462
ORF length (spliced) 342
Entry clone length 462
No. of intron 1
Sequence status Finished
Sequence results 160A:deletion
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC890.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCCAACACTAAGAAGAG
Rev primer name SPAC890.08.Rv
Rev primer SEQ AGAAAGCTGGGTATTCCTCAACAACGGTGGTC
Amino acid length 113
Molecular weight 13.2
Isoelectric point (calc.) 10.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 1 images) See all images

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