Gene name |
SPAC1556.05c |
Gene ID |
04/C10 |
Gene synonyms/obsolete |
|
Gene product |
CGR1 family; involved
in rRNA processing; conserved fungal protein; involved in
ribosome biogenesis and assembly; predicted coiled-coil
region |
Entry clone |
Cloned |
ORF length (unspliced) |
470 |
ORF length (spliced) |
336 |
Entry clone length |
470 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1556.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTAATGGAACTAAGGG |
Rev primer name |
SPAC1556.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCGCTCTTTCAACATTTTG |
Amino acid length |
111 |
Molecular weight |
13.5 |
Isoelectric point (calc.) |
11.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
95/97 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |