Gene name |
SPAC57A10.06 |
Gene ID |
04/E07 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan; has transcript profile on microarray |
Entry clone |
Cloned |
ORF length (unspliced) |
486 |
ORF length (spliced) |
402 |
Entry clone length |
486 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC57A10.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACGTTGGTGATTTGAA |
Rev primer name |
SPAC57A10.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGTTTCATCCTTTGTAATT |
Amino acid length |
133 |
Molecular weight |
14.6 |
Isoelectric point (calc.) |
4.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |