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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC887.07
Gene ID 04/E11
Gene synonyms/obsolete
Gene product mitochondrial ribosomal protein l14
Entry clone Cloned
ORF length (unspliced) 487
ORF length (spliced) 381
Entry clone length 487
No. of intron 2
Sequence status Finished
Sequence results (-5)C:deletion
Comments 5' terminus is frameshifted.
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC887.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATCGGACTTAAAGGTAT
Rev primer name SPBC887.07.Rv
Rev primer SEQ AGAAAGCTGGGTAGATGGTTCGTGGAGCAAGA
Amino acid length 126
Molecular weight 13.6
Isoelectric point (calc.) 11
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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