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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3H8.03
Gene ID 04/F01
Gene synonyms/obsolete
Gene product mitochondrial ribosomal protein
Entry clone Cloned
ORF length (unspliced) 489
ORF length (spliced) 318
Entry clone length 489
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3H8.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGATCATCCTTAAAGCC
Rev primer name SPAC3H8.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAACCTTGGTCTTGTAAC
Amino acid length 105
Molecular weight 12
Isoelectric point (calc.) 11.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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