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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC965.14c
Gene ID 04/F08
Gene synonyms/obsolete
Gene product cytidine and deoxycytidylate deaminase; tRNA-specific adenosine deaminase; involved in tRNA processing; zinc-binding protein
Entry clone Cloned
ORF length (unspliced) 489
ORF length (spliced)
Entry clone length 489
No. of intron 0
Sequence status Finished
Sequence results 169A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC965.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCAGCACTGAACTTTC
Rev primer name SPCC965.14.Rv
Rev primer SEQ AGAAAGCTGGGTATCTTCCTCCATCCCAAAAG
Amino acid length 162
Molecular weight 17.8
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 1 images) See all images

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