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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC18.16c
Gene ID 04/G03
Gene synonyms/obsolete fmn1
Gene product riboflavin kinase; FMN biosynthesis
Entry clone Cloned
ORF length (unspliced) 492
ORF length (spliced)
Entry clone length 492
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC18.16.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACAGTTAATTTAGAAGA
Rev primer name SPCC18.16.Rv
Rev primer SEQ AGAAAGCTGGGTATACCTTGAAAAATGGGTCC
Amino acid length 163
Molecular weight 18.9
Isoelectric point (calc.) 7.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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