Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC25B8.01
Gene ID 04/H03
Gene synonyms/obsolete SPAC26H5.15
Gene product MAPR family steroid-binding protein; involved in DNA repair; involved in DNA damage response; involved in sterol metabolism
Entry clone Cloned
ORF length (unspliced) 501
ORF length (spliced)
Entry clone length 501
No. of intron 0
Sequence status Finished
Sequence results 475A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC25B8.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCATCTACACAAGTTGT
Rev primer name SPAC25B8.01.Rv
Rev primer SEQ AGAAAGCTGGGTAATTATGCGCAACTTTTTCT
Amino acid length 166
Molecular weight 18.8
Isoelectric point (calc.) 4.6
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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