Gene name |
SPAC25B8.01 |
Gene ID |
04/H03 |
Gene synonyms/obsolete |
SPAC26H5.15 |
Gene product |
MAPR family
steroid-binding protein; involved in DNA repair; involved in
DNA damage response; involved in sterol metabolism |
Entry clone |
Cloned |
ORF length (unspliced) |
501 |
ORF length (spliced) |
|
Entry clone length |
501 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
475A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC25B8.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCATCTACACAAGTTGT |
Rev primer name |
SPAC25B8.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTATGCGCAACTTTTTCT |
Amino acid length |
166 |
Molecular weight |
18.8 |
Isoelectric point (calc.) |
4.6 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |