Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1450.06c
Gene ID 04/H09
Gene synonyms/obsolete
Gene product putative glutaredoxin-like protein; arsenate reductase (glutaredoxin) activity; glutaredoxin; monothiol glutaredoxin; thioltransferase
Entry clone Cloned
ORF length (unspliced) 501
ORF length (spliced)
Entry clone length 501
No. of intron 0
Sequence status Finished
Sequence results 128A:G / 171A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1450.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTAATAGAAAGATTTT
Rev primer name SPCC1450.06.Rv
Rev primer SEQ AGAAAGCTGGGTAGTCGGTAGGTAAAATTAAA
Amino acid length 166
Molecular weight 18.3
Isoelectric point (calc.) 4.7
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LITSLIISLLL
Localization (YFP) ER; Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss, DeltaVision

Image information
YFP 3 images) See all images

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