| Gene name |
SPAC26A3.08 |
| Gene ID |
05/A04 |
| Gene synonyms/obsolete |
smb1; smb |
| Gene product |
small nuclear
ribonucleoprotein associated protein B; putative pre-mRNA
splicing factor; complexed with Cdc5p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
509 |
| ORF length (spliced) |
444 |
| Entry clone length |
509 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC26A3.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTATGTGGCATTTGGAA |
| Rev primer name |
SPAC26A3.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACAGGACGAAAACCCGGG |
| Amino acid length |
147 |
| Molecular weight |
15.4 |
| Isoelectric point (calc.) |
10.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |